• Clinically-proven ingredients
  • Mixes well and tastes good
  • Contains proprietary blends
  • Contains unproven ingredient
7.6Overall Score
Reader Rating: (1 Vote)

EST Nutrition has rolled out a new pre-workout: MethylMass 2.0. Supposedly an improvement on the original formula, MethylMass 2.0 is advertised as a cell volumizer, energy booster, and hydration supplements.

I’m immediately skeptical of pre-workout products. There are so many different blends, stimulants, and ingredients that it’s often hard to determine effectiveness. I’ve also had bad experiences with pre-workouts, so I’m extra cautious.

Ultimately, the true test of any supplement lies in the ingredients, so I took a look at what’s under the cap of MethylMass 2.0.

The Ingredients

Vitamin B3

The B vitamins are commonly called the “energy vitamins” because of their role in metabolism. They allow the body to break down carbohydrates and protein for energy.

Vitamin B3, or niacin, performs 2 specific functions relevant to pre-workout. It carries hydrogen during the electron transport chain, which is a major source of energy. This provides more energy during exercise.

Niacin’s second role is that of a vasodilator and nitric oxide booster. It relaxes and expands blood vessels and stimulate nitric oxide production. This allows for greater blood flow to muscles, soaking them in oxygen and compound necessary for growth. [1]

Vasodilation and nitric oxide production are commonly called the “workout pump.” It is a feeling as strength and power during high-intensity exercise.

Vitamin B6

Another energy vitamin, vitamin B6 plays a role in amino acid metabolism. By enhancing protein absorption rate, it improves muscle regeneration.

Studies show vitamin B6 also acts as a precursor to testosterone, which could improve muscle gain. [2]

E-Bolish Technology

This is a proprietary blend, unique to MethylMass 2.0. Proprietary blends are difficult to analyze because they do not list the individual amounts of each ingredient.

However, this case is slightly odd because the E-Bolish Technology blend contains only 1 ingredient: Androsta-3,5 Diene 7,17 Dione.

Androsta-3,5 Diene 7,17 Dione is an aromatase inhibitor and metabolite of 7 Keto DHEA. Like other aromatase inhibitors, it supposedly decreases estrogen conversion and may increase testosterone levels.

Another name for the compounds is “Arimistane,” which is more commonly listed on other supplements

While it looks appealing, the National Library of Medicine has absolutely no listed studies, animal, en vitro, or human, for Androsta-3,5 Diene 7,17 Dione. It may work, but we have no proof or research to back it up.

Methyl Mass 2.0 Complex

This proprietary blends contains a laundry list of more than half a dozen ingredients. The main players seem to be Betaine, Beet Root, Creatine, Agmatine, and Coconut water.

Betaine has been shown to improve muscle protein synthesis and athletic performance. [3]

Beet root improves nitric oxide production, further enhancing the workout pump. [4]

Creatine improves post-workout recovery, strength, and muscle growth ability. [5]

Agmatine is another vasodilator and nitric oxide booster, improving muscle power. [6]

Coconut water contains electrolytes to balance hydration. [7]

Neuro-Focus Complex

This last blend contains caffeine and acacia rigidula. Caffeine is well known and widely used as a focus enhancer and energy booster.

Acacia rigidula is less well-known than caffeine, but studies show it is an amphetamine-like substance that increases energy and focus. [8]

Price and Availability

Because it has only recently been released, MethylMass 2.0 is only available from the official EST Nutrition website and the VitaminShoppe. It costs around $35 for 30 servings.

I expect EST Nutritional will soon roll out the supplement more widely, giving us the opportunity to purchase it for less than this initial retail cost.

Should I Try It?

I’m torn on MethylMass 2.0. It has a lot of ingredients, and I’m always wary of proprietary blends hiding amounts. However, most of the ingredients are backed by clinical studies.

If you liked the original MethylMass or are looking for a new pre-workout, I recommend trying MethylMass 2.0. For the rest of us, I recommend waiting until the price drops to try this new supplement.


[1] “Niacin.” PubChem – National Center for Biotechnology Information.

[2] “B Vitmains.” Medline Plus – National Institutes of Health.

[3] Cholewa JM, et al. “Effects of betaine on body composition, performance, and homocysteine thiolactone.” Journal of the International Society of Sports Nutrition. 2013 Aug 22;10(1):39.

[4] Zeling Cao, et al. “Nitrite enhances RBC hypoxic ATP synthesis and the release of ATP into the vasculature: a new mechanism for nitrite-induced vasodilation.” American Journal of Physiology. 2009 October; 297(4): H1494–H1503

[5] Odland, L. Maureen et al. “Effect of oral creatine supplementation on muscle [PCr] and short-term maximum power output.” Medicine &Science in Sports & Exercise. (1997). 29(2):216-219.

[6] Wu G, Morris SM, “Arginine metabolism: nitric oxide and beyond.” Journal of Biochemistry. 1998 November 15; 336(Pt 1): 1–17.

[7] Zelman KM. “The Truth About Coconut Water. WebMD.

[8] Jacobs PL. “Acute physiological effects of the commercially available weight loss/energy product, Fastin-XR®, in contrast with the individual effects of caffeine and acacia rigidula.” Journal of the International Society of Sports Nutrition. 2012; 9(Suppl 1): P10

About The Author

Brian E. is from southern California and has worked for names like GNC and Vitamin Shoppe as a supplement expert and consultant. He currently lives in Utah and is studying marketing.

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